I-formaldehyde ne-glutaraldehydewomabili ama-ejenti amakhemikhali asetshenziswa njengama-ejenti axhumanisayo ezinhlelweni ezihlukene, ikakhulukazi emikhakheni yebhayoloji, ikhemistri, nesayensi yezinto. Nakuba zisebenzisa izinjongo ezifanayo ekuxhumaniseni ama-biomolecules nokugcina izibonelo zezinto eziphilayo, zinezakhiwo zamakhemikhali ezihlukile, ukusebenza kabusha, ubuthi, kanye nezicelo.
Ukufana:
Ama-Crosslinking Agents: Kokubili i-formaldehyde kanyeI-glutaraldehyde iyi-aldehyde, okusho ukuthi baneqembu le-carbonyl (-CHO) ekugcineni kwesakhiwo sabo samangqamuzana. Umsebenzi wabo oyinhloko uwukwenza izibopho ezihlangene phakathi kwamaqembu asebenzayo ama-biomolecules, okuholela ekuxhumaneni. I-Crosslinking ibalulekile ekusimamiseni ukwakheka kwamasampula ebhayoloji, okuwenza aqine futhi amelane nokuwohloka.
Izicelo Ze-Biomedical: Kokubili i-formaldehyde ne-glutaraldehyde zithola ukusetshenziswa okubalulekile emkhakheni we-biomedical. Ngokuvamile zisetshenziselwa ukulungiswa kwezicubu nokulondolozwa ezifundweni ze-histology kanye ne-pathology. Izicubu ezixhunyanisiwe zigcina ubuqotho bazo besakhiwo futhi zingasetshenzwa ngokuqhubekayo ngezinjongo ezihlukahlukene zokuhlaziya nokuxilonga.
Ukulawula Amagciwane: Womabili ama-ejenti anezindawo zokulwa namagciwane, okuwenza abe wusizo ezinhlelweni zokubulala amagciwane kanye nezinqubo zokuvala inzalo. Angakwazi ukwenza amagciwane, amagciwane, kanye nesikhunta angasebenzi, anciphise ubungozi bokungcola ezindaweni zaselabhorethri namathuluzi ezokwelapha.
Izicelo zezimboni: Kokubili i-formaldehyde kanyei-glutaraldehydezisetshenziswa ezinhlobonhlobo zezimboni. Asetshenziswa ekukhiqizeni izinto zokunamathisela, ama-resin, nama-polymers, kanye nezimboni zesikhumba nezindwangu.
Umehluko:
Isakhiwo Samakhemikhali: Umehluko oyinhloko phakathi kwe-formaldehyde ne-glutaraldehyde usezakhiweni zawo zamangqamuzana. I-Formaldehyde (CH2O) iyi-aldehyde elula kunazo zonke, eyakhiwe i-athomu yekhabhoni eyodwa, ama-athomu amabili e-hydrogen, ne-athomu eyodwa yomoya-mpilo. I-Glutaraldehyde (C5H8O2), ngakolunye uhlangothi, i-aliphatic aldehyde eyinkimbinkimbi, ehlanganisa ama-athomu e-carbon amahlanu, ama-athomu e-hydrogen ayisishiyagalombili, nama-athomu amabili omoya-mpilo.
Ukusebenza kabusha: I-Glutaraldehyde ivamise ukusebenza kakhulu kune-formaldehyde ngenxa yochungechunge olude lwekhabhoni. Ukuba khona kwama-athomu ekhabhoni amahlanu ku-glutaraldehyde kuyivumela ukuthi ihlanganise amabanga amade phakathi kwamaqembu asebenzayo kuma-biomolecules, okuholela ekuxhumaneni okusheshayo nangokuphumelelayo.
I-Crosslinking Efficiency: Ngenxa ye-reactivity ephezulu, i-glutaraldehyde ivamise ukusebenza ngempumelelo ekuxhumaniseni ama-biomolecules amakhulu, njengamaprotheni nama-enzyme. I-formaldehyde, ngenkathi isenamandla okuxhumanisa, ingadinga isikhathi esengeziwe noma ukugxila okuphezulu ukuze kuzuzwe imiphumela eqhathanisekayo nama-molecule amakhulu.
Ubuthi: I-Glutaraldehyde yaziwa ukuthi inobuthi kakhulu kune-formaldehyde. Ukuchayeka isikhathi eside noma okubalulekile ku-glutaraldehyde kungabangela isikhumba nokucasuka kokuphefumula, futhi kuthathwa njengesinzwa, okusho ukuthi kungaholela ekungezwani komzimba kwabanye abantu. Ngokuphambene, i-formaldehyde iyi-carcinogen eyaziwa kakhulu futhi ibeka izingozi zempilo, ikakhulukazi lapho ihogelwa noma ithinta isikhumba.
Izicelo: Nakuba womabili amakhemikhali esetshenziswa ekulungiseni izicubu, avame ukuncanyelwa ngezinjongo ezihlukene. I-formaldehyde ivamise ukusetshenziselwa ukusetshenziswa okujwayelekile kwe-histological kanye nokugqumisa, kuyilapho i-glutaraldehyde ifaneleka kakhulu ukulondoloza izakhiwo zamaselula neziza ze-antigenic ku-electron microscopy kanye nezifundo ze-immunohistochemical.
Ukuzinza: I-formaldehyde iguquguquka kakhulu futhi ijwayele ukuhwamuka ngokushesha kune-glutaraldehyde. Lesi sakhiwo singathonya izidingo zokuphatha nokugcinwa kwama-agent axhumanisayo.
Kafushane, i-formaldehyde ne-glutaraldehyde babelana ngezici ezifanayo njengama-agent axhumanisayo, kodwa ahluke kakhulu ekwakhekeni kwawo kwamakhemikhali, ukusebenza kabusha, ubuthi, kanye nezinhlelo zokusebenza. Ukuqonda kahle lo mehluko kubalulekile ekukhetheni i-ejenti exhumanisayo efanele ngezinjongo ezithile kanye nokuqinisekisa ukusetshenziswa okuphephile nangempumelelo ezimweni ezehlukene zesayensi, zezokwelapha, nezimboni.
Isikhathi sokuthumela: Jul-28-2023