Yi-dihydrocoumarin ubuthi

I-Dihydrocoumarin, amakha, asetshenziswa ekudleni, futhi esetshenziswa njenge-coumarin ebambele, esetshenziswa njengokunambitheka kwezimonyo; I-Blend ukhilimu, ukhukhunathi, ukunambitheka kwesinamoni; Kusetshenziswa futhi njengokunambitheka kogwayi.

Yi-dihydrocoumarin ubuthi

I-Dihydrocoumarin ayinobuthi. I-Dihydrocoumarin ingumkhiqizo wemvelo otholakala ku-Yellow Vanilla Rhinoceros. Ilungiselelwe yi-hydrogenation ye-coumarin ekhona phambi kukaNickel Catalyst ngo-160-200 ℃ nangaphansi kwengcindezi. Ingasetshenziswa futhi njengento eluhlaza, i-hydrolyzed kusisombululo esinamanzi e-alkali ukukhiqiza i-O-hydroxyphonylpropionic acid, dehydration, okuvinjelwe okuvaliwe.

Isimo sokugcina

Kuvaliwe futhi kumnyama, okugcinwe endaweni epholile neyomile, isikhala esisesithebeni sincane ngangokunokwenzeka ngaphansi kwezimvume zokuphepha, futhi sigcwele ukuvikelwa kwe-nitrogen. Gcina endaweni yokugcina izinto epholile, enomoya opholile. Hlala kude nomlilo, amanzi. Kufanele igcinwe ngokwahlukana kusuka ku-oxidizer, ungahlanganisi isitoreji. Ifakwe izinhlobo ezihambelana nezinhlobo zomlilo.

Esifundweni se-Vitro

E-vitro enzymatic assay, i-dihydrocoumarin incike ekuqinisekisweni kokuhlushwa kwe-sirt1 (iC50 ka-208μm). Ukuncipha komsebenzi we-Sirt1 Doacetylase kwaqashelwa ngisho nasemithinimolali ye-micromolar (85 ± 5.8 no-73 ± 13.7% umsebenzi ngo-1.6μm kanye ne-8μm, ngokulandelana). I-Microtubule Sirt2 Deacetylase nayo ibivinjelwe ngendlela ebincike emthanjeni efana nethamo (iC50 ka-295μm).

Ngemuva kwamahora angama-24 okuvezwa, i-dihydrocoumarin (1-5mm) yandisa i-cytotoxicicity ku-TK6 cell lines ngendlela ethembele umthamo. I-Dihydrocoumarin (1-5mm) ikhuphuke kakhulu i-apoptosis emigqeni yeseli ye-TK6 ngendlela ethembele umthamo ngephuzu lesikhathi samahora ayi-6. Umthamo we-5mm we-dihydrocoumarin ukhuphuke kakhulu i-apoptosis ngephuzu lesikhathi samahora ayi-6 emugqeni weseli we-TK6. Ngemuva kwesikhathi sokuvezwa kwamahora angama-24, i-Dihydrocoumarin (1-5mm) yandisa i-P53 Lysine 373 kanye ne-382 acetylation ngendlela ethembele emthanjeni we-TK6 cell.