Ingabe i-dihydrocoumarin inobuthi

I-Dihydrocoumarin, iphunga elimnandi, elisetshenziswa ekudleni, liphinde lisetshenziswe njenge-coumarin esikhundleni, elisetshenziswa njenge-flavour yezimonyo; Hlanganisa ukhilimu, ukhukhunathi, ukunambitheka kwesinamoni; Isetshenziswa futhi njengesinambithelo sikagwayi.

Ingabe i-dihydrocoumarin inobuthi

I-Dihydrocoumarin ayinabo ubuthi. I-Dihydrocoumarin iwumkhiqizo wemvelo otholakala kubhejane we-vanilla ophuzi. Ilungiswa nge-hydrogenation ye-coumarin phambi kwe-nickel catalyst ku-160-200 ℃ nangaphansi kwengcindezi. Ingasetshenziswa futhi njengempahla eluhlaza, i-hydrolyzed kusisombululo se-alkaline samanzi ukukhiqiza i-o-hydroxyphenylpropionic acid, ukuphelelwa amandla kwamanzi, i-loop evaliwe etholiwe.

Isimo sesitoreji

Kuvaliwe futhi kumnyama, kugcinwe endaweni epholile neyomile, isikhala emgqonyeni sincane ngangokunokwenzeka ngaphansi kwezimvume zokuphepha, futhi sigcwele ukuvikelwa kwe-nitrogen. Gcina endaweni epholile, engenisa umoya. Hlala kude nomlilo, amanzi. Kufanele igcinwe ngokuhlukana ne-oxidizer, ungahlanganisi isitoreji. Ifakwe izinhlobonhlobo ezihambisanayo kanye nenani lamathuluzi omlilo.

Isifundo se-in vitro

I-in vitro enzymatic assay, i-dihydrocoumarin yenza ukuvinjelwa okuncike ekugxiliseni kwe-SIRT1 (IC50 ye-208μM). Ukwehliswa komsebenzi we-SIRT1 deacetylase kubonwe ngisho nakumithamo ye-micromolar (85±5.8 kanye no-73± 13.7% umsebenzi ku-1.6μM naku-8μM, ngokulandelana). I-Microtubule SIRT2 deacetylase nayo yavinjelwa ngendlela efanayo encike kumthamo (IC50 ye-295μM).

Ngemva kwamahora angu-24 okuchayeka, i-dihydrocoumarin (1-5mM) yenyusa i-cytotoxicity emigqeni yeseli ye-TK6 ngendlela encike kumthamo. I-Dihydrocoumarin (1-5mM) ikhuphule i-apoptosis emigqeni yeseli ye-TK6 ngendlela encike kumthamo endaweni yesikhathi samahora angu-6. Umthamo ongu-5mM we-dihydrocoumarin ukhuphule i-apoptosis endaweni yesikhathi samahora angu-6 kulayini weseli we-TK6. Ngemuva kwesikhathi sokuchayeka samahora angama-24, i-dihydrocoumarin (1-5mM) yenyusa i-p53 lysine 373 kanye ne-382 acetylation ngendlela encike kumthamo kumugqa weseli we-TK6.