Ingabe i-dihydrocoumarin inobuthi

I-Dihydrocoumarin, iphunga elimnandi, elisetshenziswa ekudleni, liphinde lisetshenziswe njengesithasiselo se-coumarin, lisetshenziswe njengesinambitheko sobuhle; Ukhilimu ohlanganisiwe, ukhukhunathi, ukunambitheka kwesinamoni; Liphinde lisetshenziswe njengesinambitheko sikagwayi.

Ingabe i-dihydrocoumarin inobuthi

I-Dihydrocoumarin ayinobuthi. I-Dihydrocoumarin iwumkhiqizo wemvelo otholakala ku-yellow vanilla rhinoceros. Ilungiswa nge-hydrogenation ye-coumarin lapho kukhona i-nickel catalyst ku-160-200 ℃ futhi ingaphansi kwengcindezi. Ingasetshenziswa futhi njengezinto zokusetshenziswa, ezifakwe i-hydrolyzed kusisombululo samanzi se-alkaline ukukhiqiza i-o-hydroxyphenylpropionic acid, ukuphelelwa amanzi emzimbeni, kanye ne-closed-loop etholakele.

Isimo sesitoreji

Ivaliwe futhi imnyama, igcinwe endaweni epholile neyomile, isikhala esisebhakedeni sincane ngangokunokwenzeka ngaphansi kwezimvume zokuphepha, futhi sigcwele isivikelo se-nitrogen. Gcina endaweni yokugcina izinto epholile, enomoya. Hlala kude nomlilo, amanzi. Kufanele igcinwe ngokwehlukana ne-oxidizer, ungahlanganisi indawo yokugcina. Ifakwe izinhlobo ezahlukene kanye nenani elifanele lemishini yomlilo.

Ucwaningo lwe-in vitro

Ukuhlolwa kwe-enzyme ye-in vitro, i-dihydrocoumarin ibangele ukuvinjelwa okuncike ekugxilweni kwe-SIRT1 (IC50 ye-208μM). Ukwehla komsebenzi we-deacetylase ye-SIRT1 kwabonwa ngisho nasemithanjeni ye-micromolar (umsebenzi we-85±5.8 kanye no-73± 13.7% ku-1.6μM kanye no-8μM, ngokulandelana). I-deacetylase ye-Microtubule SIRT2 nayo yavinjelwa ngendlela efanayo encike kumthamo (IC50 ye-295μM).

Ngemva kwamahora angu-24 okuchayeka, i-dihydrocoumarin (1-5mM) yandisa ubuthi be-cytotoxic emigqeni yamaseli e-TK6 ngendlela encike kumthamo. I-Dihydrocoumarin (1-5mM) yandisa i-apoptosis emigqeni yamaseli e-TK6 ngendlela encike kumthamo ngesikhathi samahora angu-6. Umthamo we-5mM we-dihydrocoumarin wandisa i-apoptosis ngesikhathi samahora angu-6 emgqeni wamaseli e-TK6. Ngemva kwesikhathi sokuchayeka samahora angu-24, i-dihydrocoumarin (1-5mM) yandisa i-p53 lysine 373 kanye ne-382 acetylation ngendlela encike kumthamo emgqeni wamaseli e-TK6.